SECURITIES AND EXCHANGE COMMISSION
Washington, D.C. 20549
____________________
SCHEDULE 13G
UNDER THE SECURITIES EXCHANGE ACT OF 1934
(Amendment No. 4)*
ACUSPHERE, INC.
___________________________________________________________
(Name of Issuer)
COMMON STOCK
___________________________________________________________
(Title of Class of Securities)
00511R870
____________________________
(CUSIP Number)
December 31, 2007
___________________________________________________________
(Date of Event Which Requires Filing of this Statement)
Check the appropriate box to designate the Rule pursuant to which
this Schedule is filed:
[ ] Rule 13d - 1(b)
[X] Rule 13d - 1(c)
[ ] Rule 13d - 1(d)
* The remainder of this cover page shall be filled out for a
reporting person's initial filing on this form with respect to the
subject class of securities, and for any subsequent amendment
containing information which would alter disclosures provided in a
prior cover page.
The information required on the remainder of this page shall
not be deemed to be "filed" for the purpose of Section 18 of the
Securities Exchange Act of 1934 ("Act") or otherwise subject to the
liabilities of that section of the Act but shall be subject to all
other provisions of the Act (however, see the Notes.)
(Continued on following page(s))
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CUSIP No. 13G 2 of 19 pages
00511R 87 0
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1 NAMES OF REPORTING PERSONS
I.R.S. IDENTIFICATION NO. OF ABOVE PERSONS
(ENTITIES ONLY):
Bank of America Corporation
56-0906609
- ---------------------------------------------------------
- ---------------------------------------------------------
2 CHECK THE APPROPRIATE BOX IF A MEMBER OF A
GROUP (See Instructions)
(a) [ ]
(b) [ ]
- ---------------------------------------------------------
- ---------------------------------------------------------
3 SEC USE ONLY
- ---------------------------------------------------------
- ---------------------------------------------------------
4 CITIZENSHIP OR PLACE OF ORGANIZATION
Delaware
- ---------------------------------------------------------
- ---------------------------------------------------------
0
5 SOLE VOTING POWER
NUMBER OF
SHARES
BENEFICIALLY
OWNED BY EACH
REPORTING
PERSON WITH
- ---------------------------------------------------------
- ---------------------------------------------------------
2,675,184*
6 SHARED VOTING POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
7 SOLE DISPOSITIVE 0
POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
8 SHARED DISPOSITIVE 3,076,377*
POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
9 AGGREGATE AMOUNT BENEFICIALLY OWNED BY EACH
REPORTING PERSON
3,076,377*
- ---------------------------------------------------------
- ---------------------------------------------------------
10 CHECK IF THE AGGREGATE AMOUNT IN ROW (9)
EXCLUDES CERTAIN SHARES (See Instructions)
[ ]
- ---------------------------------------------------------
- ---------------------------------------------------------
11 PERCENT OF CLASS REPRESENTED BY AMOUNT IN ROW
(9)
6.6%*
- ---------------------------------------------------------
- ---------------------------------------------------------
12 TYPE OF REPORTING PERSON (See Instructions)
HC
- ---------------------------------------------------------
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CUSIP No. 13G 3 of 19 pages
00511R 87 0
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1 NAMES OF REPORTING PERSONS
I.R.S. IDENTIFICATION NO. OF ABOVE PERSONS
(ENTITIES ONLY):
NB Holdings Corporation
56-1857749
- ---------------------------------------------------------
- ---------------------------------------------------------
2 CHECK THE APPROPRIATE BOX IF A MEMBER OF A
GROUP (See Instructions)
(a) [ ]
(b) [ ]
- ---------------------------------------------------------
- ---------------------------------------------------------
3 SEC USE ONLY
- ---------------------------------------------------------
- ---------------------------------------------------------
4 CITIZENSHIP OR PLACE OF ORGANIZATION
Delaware
- ---------------------------------------------------------
- ---------------------------------------------------------
0
5 SOLE VOTING POWER
NUMBER OF
SHARES
BENEFICIALLY
OWNED BY EACH
REPORTING
PERSON WITH
- ---------------------------------------------------------
- ---------------------------------------------------------
2,522,301*
6 SHARED VOTING POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
7 SOLE DISPOSITIVE 0
POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
8 SHARED DISPOSITIVE 2,906,477*
POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
9 AGGREGATE AMOUNT BENEFICIALLY OWNED BY EACH
REPORTING PERSON
2,906,477*
- ---------------------------------------------------------
- ---------------------------------------------------------
10 CHECK IF THE AGGREGATE AMOUNT IN ROW (9)
EXCLUDES CERTAIN SHARES (See Instructions)
[ ]
- ---------------------------------------------------------
- ---------------------------------------------------------
11 PERCENT OF CLASS REPRESENTED BY AMOUNT IN ROW
(9)
6.3%*
- ---------------------------------------------------------
- ---------------------------------------------------------
12 TYPE OF REPORTING PERSON (See Instructions)
CO
- ---------------------------------------------------------
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CUSIP No. 13G 4 of 19 pages
00511R 87 0
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1 NAMES OF REPORTING PERSONS
I.R.S. IDENTIFICATION NO. OF ABOVE PERSONS
(ENTITIES ONLY):
Bank of America, National Association
94-1687665
- ---------------------------------------------------------
- ---------------------------------------------------------
2 CHECK THE APPROPRIATE BOX IF A MEMBER OF A GROUP
(See Instructions)
(a) [ ]
(b) [ ]
- ---------------------------------------------------------
- ---------------------------------------------------------
3 SEC USE ONLY
- ---------------------------------------------------------
- ---------------------------------------------------------
4 CITIZENSHIP OR PLACE OF ORGANIZATION
United States
- ---------------------------------------------------------
- ---------------------------------------------------------
17,017
5 SOLE VOTING POWER
NUMBER OF
SHARES
BENEFICIALLY
OWNED BY EACH
REPORTING
PERSON WITH
- ---------------------------------------------------------
- ---------------------------------------------------------
2,318,141*
6 SHARED VOTING POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
7 SOLE DISPOSITIVE 17,017
POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
8 SHARED DISPOSITIVE 2,554,534*
POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
9 AGGREGATE AMOUNT BENEFICIALLY OWNED BY EACH
REPORTING PERSON
2,571,551*
- ---------------------------------------------------------
- ---------------------------------------------------------
10 CHECK IF THE AGGREGATE AMOUNT IN ROW (9)
EXCLUDES CERTAIN SHARES (See Instructions)
[ ]
- ---------------------------------------------------------
- ---------------------------------------------------------
11 PERCENT OF CLASS REPRESENTED BY AMOUNT IN ROW
(9)
5.5%*
- ---------------------------------------------------------
- ---------------------------------------------------------
12 TYPE OF REPORTING PERSON (See Instructions)
BK
- ---------------------------------------------------------
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CUSIP No. 13G 5 of 19 pages
00511R 87 0
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1 NAMES OF REPORTING PERSONS
I.R.S. IDENTIFICATION NO. OF ABOVE PERSONS
(ENTITIES ONLY):
Bank of America Ventures
95-6016836
- ---------------------------------------------------------
- ---------------------------------------------------------
2 CHECK THE APPROPRIATE BOX IF A MEMBER OF A GROUP
(See Instructions)
(a) [ ]
(b) [ ]
- ---------------------------------------------------------
- ---------------------------------------------------------
3 SEC USE ONLY
- ---------------------------------------------------------
- ---------------------------------------------------------
4 CITIZENSHIP OR PLACE OF ORGANIZATION
California
- ---------------------------------------------------------
- ---------------------------------------------------------
1,375,975*
5 SOLE VOTING POWER
NUMBER OF
SHARES
BENEFICIALLY
OWNED BY EACH
REPORTING
PERSON WITH
- ---------------------------------------------------------
- ---------------------------------------------------------
0
6 SHARED VOTING POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
7 SOLE DISPOSITIVE 1,375,975*
POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
8 SHARED DISPOSITIVE 0
POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
9 AGGREGATE AMOUNT BENEFICIALLY OWNED BY EACH
REPORTING PERSON
1,375,975*
- ---------------------------------------------------------
- ---------------------------------------------------------
10 CHECK IF THE AGGREGATE AMOUNT IN ROW (9)
EXCLUDES CERTAIN SHARES (See Instructions)
[ ]
- ---------------------------------------------------------
- ---------------------------------------------------------
11 PERCENT OF CLASS REPRESENTED BY AMOUNT IN ROW
(9)
3.0%
- ---------------------------------------------------------
- ---------------------------------------------------------
12 TYPE OF REPORTING PERSON (See Instructions)
CO
- ---------------------------------------------------------
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CUSIP No. 13G 6 of 19 pages
00511R 87 0
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1 NAMES OF REPORTING PERSONS
I.R.S. IDENTIFICATION NO. OF ABOVE PERSONS
(ENTITIES ONLY):
BancBoston Ventures, Inc.
04-6013165
- ---------------------------------------------------------
- ---------------------------------------------------------
2 CHECK THE APPROPRIATE BOX IF A MEMBER OF A GROUP
(See Instructions)
(a) [ ]
(b) [ ]
- ---------------------------------------------------------
- ---------------------------------------------------------
3 SEC USE ONLY
- ---------------------------------------------------------
- ---------------------------------------------------------
4 CITIZENSHIP OR PLACE OF ORGANIZATION
Massachusetts
- ---------------------------------------------------------
- ---------------------------------------------------------
292,554
5 SOLE VOTING POWER
NUMBER OF
SHARES
BENEFICIALLY
OWNED BY EACH
REPORTING
PERSON WITH
- ---------------------------------------------------------
- ---------------------------------------------------------
0
6 SHARED VOTING POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
7 SOLE DISPOSITIVE 292,554
POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
8 SHARED DISPOSITIVE 0
POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
9 AGGREGATE AMOUNT BENEFICIALLY OWNED BY EACH
REPORTING PERSON
292,554
- ---------------------------------------------------------
- ---------------------------------------------------------
10 CHECK IF THE AGGREGATE AMOUNT IN ROW (9)
EXCLUDES CERTAIN SHARES (See Instructions)
[ ]
- ---------------------------------------------------------
- ---------------------------------------------------------
11 PERCENT OF CLASS REPRESENTED BY AMOUNT IN ROW (9)
0.6%
- ---------------------------------------------------------
- ---------------------------------------------------------
12 TYPE OF REPORTING PERSON (See Instructions)
CO
- ---------------------------------------------------------
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CUSIP No. 13G 7 of 19 pages
00511R 87 0
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1 NAMES OF REPORTING PERSONS
I.R.S. IDENTIFICATION NO. OF ABOVE PERSONS
(ENTITIES ONLY):
Columbia Management Group, LLC
94-1687665
- ---------------------------------------------------------
- ---------------------------------------------------------
2 CHECK THE APPROPRIATE BOX IF A MEMBER OF A GROUP
(See Instructions)
(a) [ ]
(b) [ ]
- ---------------------------------------------------------
- ---------------------------------------------------------
3 SEC USE ONLY
- ---------------------------------------------------------
- ---------------------------------------------------------
4 CITIZENSHIP OR PLACE OF ORGANIZATION
Delaware
- ---------------------------------------------------------
- ---------------------------------------------------------
0
5 SOLE VOTING POWER
NUMBER OF
SHARES
BENEFICIALLY
OWNED BY EACH
REPORTING
PERSON WITH
- ---------------------------------------------------------
- ---------------------------------------------------------
649,307
6 SHARED VOTING POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
7 SOLE DISPOSITIVE 0
POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
8 SHARED DISPOSITIVE 885,700
POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
9 AGGREGATE AMOUNT BENEFICIALLY OWNED BY EACH
REPORTING PERSON
885,700
- ---------------------------------------------------------
- ---------------------------------------------------------
10 CHECK IF THE AGGREGATE AMOUNT IN ROW (9)
EXCLUDES CERTAIN SHARES (See Instructions)
[ ]
- ---------------------------------------------------------
- ---------------------------------------------------------
11 PERCENT OF CLASS REPRESENTED BY AMOUNT IN ROW (9)
1.9%
- ---------------------------------------------------------
- ---------------------------------------------------------
12 TYPE OF REPORTING PERSON (See Instructions)
CO
- ---------------------------------------------------------
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CUSIP No. 13G 8 of 19 pages
00511R 87 0
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1 NAMES OF REPORTING PERSONS
I.R.S. IDENTIFICATION NO. OF ABOVE PERSONS
(ENTITIES ONLY):
Columbia Management Advisors, LLC
94-1687665
- ---------------------------------------------------------
- ---------------------------------------------------------
2 CHECK THE APPROPRIATE BOX IF A MEMBER OF A GROUP
(See Instructions)
(a) [ ]
(b) [ ]
- ---------------------------------------------------------
- ---------------------------------------------------------
3 SEC USE ONLY
- ---------------------------------------------------------
- ---------------------------------------------------------
4 CITIZENSHIP OR PLACE OF ORGANIZATION
Delaware
- ---------------------------------------------------------
- ---------------------------------------------------------
649,307
5 SOLE VOTING POWER
NUMBER OF
SHARES
BENEFICIALLY
OWNED BY EACH
REPORTING
PERSON WITH
- ---------------------------------------------------------
- ---------------------------------------------------------
0
6 SHARED VOTING POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
7 SOLE DISPOSITIVE 882,900
POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
8 SHARED DISPOSITIVE 2,800
POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
9 AGGREGATE AMOUNT BENEFICIALLY OWNED BY EACH
REPORTING PERSON
885,700
- ---------------------------------------------------------
- ---------------------------------------------------------
10 CHECK IF THE AGGREGATE AMOUNT IN ROW (9)
EXCLUDES CERTAIN SHARES (See Instructions)
[ ]
- ---------------------------------------------------------
- ---------------------------------------------------------
11 PERCENT OF CLASS REPRESENTED BY AMOUNT IN ROW (9)
1.9%
- ---------------------------------------------------------
- ---------------------------------------------------------
12 TYPE OF REPORTING PERSON (See Instructions)
IA
- ---------------------------------------------------------
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CUSIP No. 13G 9 of 19 pages
00511R 87 0
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1 NAMES OF REPORTING PERSONS
I.R.S. IDENTIFICATION NO. OF ABOVE PERSONS
(ENTITIES ONLY):
BankAmerica Investment Corporation
36-3101574
- ---------------------------------------------------------
- ---------------------------------------------------------
2 CHECK THE APPROPRIATE BOX IF A MEMBER OF A
GROUP (See Instructions)
(a) [ ]
(b) [ ]
- ---------------------------------------------------------
- ---------------------------------------------------------
3 SEC USE ONLY
- ---------------------------------------------------------
- ---------------------------------------------------------
4 CITIZENSHIP OR PLACE OF ORGANIZATION
Delaware
- ---------------------------------------------------------
- ---------------------------------------------------------
152,883
5 SOLE VOTING POWER
NUMBER OF
SHARES
BENEFICIALLY
OWNED BY EACH
REPORTING
PERSON WITH
- ---------------------------------------------------------
- ---------------------------------------------------------
0
6 SHARED VOTING POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
7 SOLE DISPOSITIVE 152,883
POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
8 SHARED DISPOSITIVE 0
POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
9 AGGREGATE AMOUNT BENEFICIALLY OWNED BY EACH
REPORTING PERSON
152,883
- ---------------------------------------------------------
- ---------------------------------------------------------
10 CHECK IF THE AGGREGATE AMOUNT IN ROW (9)
EXCLUDES CERTAIN SHARES (See Instructions)
[ ]
- ---------------------------------------------------------
- ---------------------------------------------------------
11 PERCENT OF CLASS REPRESENTED BY AMOUNT IN ROW
(9)
0.3%
- ---------------------------------------------------------
- ---------------------------------------------------------
12 TYPE OF REPORTING PERSON (See Instructions)
CO
- ---------------------------------------------------------
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CUSIP No. 13G 10 of 19
00511R 87 0 pages
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1 NAMES OF REPORTING PERSONS
I.R.S. IDENTIFICATION NO. OF ABOVE PERSONS
(ENTITIES ONLY):
Bank of America Capital Advisors LLC
36-3009841
- ---------------------------------------------------------
- ---------------------------------------------------------
2 CHECK THE APPROPRIATE BOX IF A MEMBER OF A
GROUP (See Instructions)
(a) [ ]
(b) [ ]
- ---------------------------------------------------------
- ---------------------------------------------------------
3 SEC USE ONLY
- ---------------------------------------------------------
- ---------------------------------------------------------
4 CITIZENSHIP OR PLACE OF ORGANIZATION
Delaware
- ---------------------------------------------------------
- ---------------------------------------------------------
187,143
5 SOLE VOTING POWER
NUMBER OF
SHARES
BENEFICIALLY
OWNED BY EACH
REPORTING
PERSON WITH
- ---------------------------------------------------------
- ---------------------------------------------------------
0
6 SHARED VOTING POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
7 SOLE DISPOSITIVE 187,143
POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
8 SHARED DISPOSITIVE 0
POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
9 AGGREGATE AMOUNT BENEFICIALLY OWNED BY EACH
REPORTING PERSON
187,143
- ---------------------------------------------------------
- ---------------------------------------------------------
10 CHECK IF THE AGGREGATE AMOUNT IN ROW (9)
EXCLUDES CERTAIN SHARES (See Instructions)
[ ]
- ---------------------------------------------------------
- ---------------------------------------------------------
11 PERCENT OF CLASS REPRESENTED BY AMOUNT IN ROW
(9)
0.4%
- ---------------------------------------------------------
- ---------------------------------------------------------
12 TYPE OF REPORTING PERSON (See Instructions)
IA
- ---------------------------------------------------------
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CUSIP No. 13G 11 of 19
00511R 87 0 pages
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1 NAMES OF REPORTING PERSONS
I.R.S. IDENTIFICATION NO. OF ABOVE PERSONS
(ENTITIES ONLY):
United States Trust Company, NA
13-3818954
- ---------------------------------------------------------
- ---------------------------------------------------------
2 CHECK THE APPROPRIATE BOX IF A MEMBER OF A GROUP
(See Instructions)
(a) [ ]
(b) [ ]
- ---------------------------------------------------------
- ---------------------------------------------------------
3 SEC USE ONLY
- ---------------------------------------------------------
- ---------------------------------------------------------
4 CITIZENSHIP OR PLACE OF ORGANIZATION
United States
- ---------------------------------------------------------
- ---------------------------------------------------------
0
5 SOLE VOTING POWER
NUMBER OF
SHARES
BENEFICIALLY
OWNED BY EACH
REPORTING
PERSON WITH
- ---------------------------------------------------------
- ---------------------------------------------------------
0
6 SHARED VOTING POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
7 SOLE DISPOSITIVE 164,800
POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
8 SHARED DISPOSITIVE 0
POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
9 AGGREGATE AMOUNT BENEFICIALLY OWNED BY EACH
REPORTING PERSON
164,800
- ---------------------------------------------------------
- ---------------------------------------------------------
10 CHECK IF THE AGGREGATE AMOUNT IN ROW (9)
EXCLUDES CERTAIN SHARES (See Instructions)
[ ]
- ---------------------------------------------------------
- ---------------------------------------------------------
11 PERCENT OF CLASS REPRESENTED BY AMOUNT IN ROW (9)
0.4%
- ---------------------------------------------------------
- ---------------------------------------------------------
12 TYPE OF REPORTING PERSON (See Instructions)
BK
- ---------------------------------------------------------
Item 1(a). Name of Issuer:
Acusphere, Inc.
Item 1(b). Address of Issuer's Principal Executive Offices:
500 Arsenal Street
Watertown, Massachusetts 02472
Item 2(a). Name of Person Filing:
Bank of America Corporation
NB Holdings Corporation
Bank of America, National Association
BancBoston Ventures, Inc.
Banc of America Ventures
BankAmerica Investment Corporation
Columbia Management Group, LLC
Columbia Management Advisors, LLC
Bank of America Capital Advisors LLC
United States Trust Company, N.A.
Item 2(b). Address of Principal Business Office or, if None,
Residence:
The address for Bank of America Ventures and BankAmerica
Investment Corporation is 950 Tower Lane, Suite 700, Foster
City, CA 94404.
Each other Reporting Person has its or his principal
business office at 100 North Tryon Street, Floor 25, Bank
of America Corporate Center, Charlotte, NC 28255.
Item 2(c). Citizenship:
Bank of America Corporation Delaware
NB Holdings Corporation Delaware
Bank of America, National Association United States
BancBoston Ventures, Inc. Massachusetts
Banc of America Ventures California
BankAmerica Investment Corporation Delaware
Columbia Management Group, LLC Delaware
Columbia Management Advisors, LLC Delaware
Bank of America Capital Advisors LLC Delaware
United States Trust Company, N.A. United States
Item 2(d). Title of Class of Securities: Common Stock
Item 2(e). CUSIP Number: 00511R870
Item 3. If This Statement is Filed Pursuant to Rule 13d-1(b), or
13d-2(b) or (c), Check Whether the Person Filing is a:
(a) [ ] Broker or dealer registered under Section 15 of
the Exchange Act.
(b) [ ] Bank as defined in Section 3(a)(6) of the
Exchange Act.
(c) [ ] Insurance company as defined in Section 3(a)(19)
of the Exchange Act.
(d) [ ] Investment company registered under Section 8
of the Investment Company Act.
(e) [ ] An investment adviser in accordance with
Rule 13d-1(b)(1)(ii)(E).
(f) [ ] An employee benefit plan or endowment fund in
accordance with Rule 13d-1(b)(1)(ii)(F).
(g) [ ] A parent holding company or control person in
accordance with Rule 13d-1(b)(1)(ii)(G).
(h) [ ] A savings association as defined in Section 3(b)
of the Federal Deposit Insurance Act.
(i) [ ] A church plan that is excluded from the
definition of an investment company under Section
3(c)(14) of the Investment Company Act.
(j) [ ] Group, in accordance with Rule 13d-1(b)(1)(ii)(J).
If this statement is filed pursuant to Rule 13d-1(c), check
this box. [x]
Item 4. Ownership:
With respect to the beneficial ownership of the reporting
person, see Items 5 through 11 of the cover pages to this
Schedule 13G, which are incorporated herein by reference.
Item 5. Ownership of Five Percent or Less of a Class:
If this statement is being filed to report the fact that as
of the date hereof the reporting person has ceased to be
the beneficial owner of more than five percent of the class
of securities, check the following [ ].
Item 6. Ownership of More than Five Percent on Behalf of Another
Person:
Not applicable.
Item 7. Identification and Classification of the Subsidiary which
Acquired the Security Being Reported on By the Parent
Holding Company:
Not applicable.
Item 8. Identification and Classification of Members of the Group:
Not applicable.
Item 9. Notice of Dissolution of Group:
Not applicable.
Item 10. Certification:
By signing below each of the undersigned certifies that, to the
best of such undersigned's knowledge and belief, the securities
referred to above were not acquired and are not for the purpose of
or with the effect of changing or influencing the control of the
issuer of the securities and were not acquired and are not held in
connection with or as a participant in any transaction having that
purpose or effect.
SIGNATURE
After reasonable inquiry and to the best of the undersigned's
knowledge and belief, the undersigned certify that the information
set forth in this statement is true, complete and correct.
Dated: February 5, 2008
BANK OF AMERICA CORPORATION
NB HOLDINGS CORPORATION
BANK OF AMERICA, NATIONAL ASSOCIATION
By: /s/ Lucille E. Reymann
Lucille E. Reymann
Senior Vice President
BANKAMERICA INVESTMENT CORPORATION
BANK OF AMERICA VENTURES
By: /s/ M. Ann O'Brien
M. Ann O'Brien
Managing Director
BANCBOSTON VENTURES, INC.
By: /s/ Maia D. Heymann
Maia D. Heymann
Managing Director
COLUMBIA MANAGEMENT GROUP, LLC
COLUMBIA MANAGEMENT ADVISORS, LLC
By: /s/ Michael A. Jones
Michael A. Jones
President
BANK OF AMERICA CAPITAL ADVISORS LLC
By: /s/ Steven Suss
Steven Suss
Managing Director
UNITED STATES TRUST COMPANY, N.A.
By: /s/ Michael Murphy
____________________
Michael Murphy
Senior Vice President
Exhibit 99.1
JOINT FILING AGREEMENT
The undersigned hereby agree that they are filing this
statement jointly pursuant to Rule 13d-1(k)(1). Each of them is
responsible for the timely filing of such Schedule 13G and any
amendments thereto, and for the completeness and accuracy of the
information concerning such person contained therein; but none of
them is responsible for the completeness or accuracy of the
information concerning the other persons making the filing, unless
such person knows or has reason to believe that such information is
inaccurate.
In accordance with Rule 13d-1(k)(1) promulgated under the
Securities and Exchange Act of 1934, as amended, the undersigned
hereby agree to the joint filing with each other on behalf of each
of them of to such a statement on Schedule 13G with respect to the
common stock of Acusphere, Inc. beneficially owned by each of them.
This Joint Filing Agreement shall be included as an exhibit to such
Schedule 13G.
Dated: February 5, 2008
BANK OF AMERICA CORPORATION
NB HOLDINGS CORPORATION
BANK OF AMERICA, NATIONAL ASSOCIATION
By: /s/ Lucille E. Reymann
Lucille E. Reymann
Senior Vice President
BANKAMERICA INVESTMENT CORPORATION
BANK OF AMERICA VENTURES
By: /s/ M. Ann O'Brien
M. Ann O'Brien
Managing Director
BANCBOSTON VENTURES, INC.
By: /s/ Maia D. Heymann
Maia D. Heymann
Managing Director
COLUMBIA MANAGEMENT GROUP, LLC
COLUMBIA MANAGEMENT ADVISORS, LLC
By: /s/ Michael A. Jones
_______________________
Michael A. Jones
President
BANK OF AMERICA CAPITAL ADVISORS LLC
By: /s/ Steven Suss
___________________
Steven Suss
Managing Director
UNITED STATES TRUST COMPANY, N.A.
By: /s/ Michael Murphy
____________________
Michael Murphy
Senior Vice President