SECURITIES AND EXCHANGE COMMISSION
Washington, D.C. 20549
____________________
SCHEDULE 13G
UNDER THE SECURITIES EXCHANGE ACT OF 1934
(Amendment No. 1)*
APPLIED NEUROSOLUTIONS INC
___________________________________________________________
(Name of Issuer)
COMMON STOCK
___________________________________________________________
(Title of Class of Securities)
03823Y104
______________________________
(CUSIP Number)
December 31, 2007
___________________________________________________________
(Date of Event Which Requires Filing of this Statement)
Check the appropriate box to designate the Rule pursuant to which
this Schedule is filed:
[X] Rule 13d - 1(b)
[ ] Rule 13d - 1(c)
[ ] Rule 13d - 1(d)
* The remainder of this cover page shall be filled out for a
reporting person's initial filing on this form with respect to the
subject class of securities, and for any subsequent amendment
containing information which would alter disclosures provided in a
prior cover page.
The information required on the remainder of this page shall
not be deemed to be "filed" for the purpose of Section 18 of the
Securities Exchange Act of 1934 ("Act") or otherwise subject to the
liabilities of that section of the Act but shall be subject to all
other provisions of the Act (however, see the Notes.)
- ----------------- --------------
CUSIP No 13G Page 4 of 9
03823Y104 Pages
- ----------------- --------------
- ---------------------------------------------------------
1 NAMES OF REPORTING PERSONS
I.R.S. IDENTIFICATION NO. OF ABOVE PERSONS
(ENTITIES ONLY):
Bank of America Corporation
56-0906609
- ---------------------------------------------------------
- ---------------------------------------------------------
2 CHECK THE APPROPRIATE BOX IF A MEMBER OF A GROUP
(See Instructions)
(a) [ ]
(b) [ ]
- ---------------------------------------------------------
- ---------------------------------------------------------
3 SEC USE ONLY
- ---------------------------------------------------------
- ---------------------------------------------------------
4 CITIZENSHIP OR PLACE OF ORGANIZATION
Delaware
- ---------------------------------------------------------
- ---------------------------------------------------------
0
5 SOLE VOTING POWER
NUMBER OF
SHARES
BENEFICIALLY
OWNED BY EACH
REPORTING
PERSON WITH
- ---------------------------------------------------------
- ---------------------------------------------------------
0
6 SHARED VOTING POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
7 SOLE DISPOSITIVE 0
POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
8 SHARED DISPOSITIVE 8,207,780
POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
9 AGGREGATE AMOUNT BENEFICIALLY OWNED BY EACH
REPORTING PERSON
8,207,780
- ---------------------------------------------------------
- ---------------------------------------------------------
10 CHECK IF THE AGGREGATE AMOUNT IN ROW (9)
EXCLUDES CERTAIN SHARES (See Instructions)
[ ]
- ---------------------------------------------------------
- ---------------------------------------------------------
11 PERCENT OF CLASS REPRESENTED BY AMOUNT IN ROW (9)
6.31%
- ---------------------------------------------------------
- ---------------------------------------------------------
12 TYPE OF REPORTING PERSON (See Instructions)
HC
- ---------------------------------------------------------
- ---------------------------------------------------------
1 NAMES OF REPORTING PERSONS
I.R.S. IDENTIFICATION NO. OF ABOVE PERSONS
(ENTITIES ONLY):
NB Holdings Corporation
56-1857749
- ---------------------------------------------------------
- ---------------------------------------------------------
2 CHECK THE APPROPRIATE BOX IF A MEMBER OF A GROUP
(See Instructions)
(a) [ ]
(b) [ ]
- ---------------------------------------------------------
- ---------------------------------------------------------
3 SEC USE ONLY
- ---------------------------------------------------------
- ---------------------------------------------------------
4 CITIZENSHIP OR PLACE OF ORGANIZATION
Delaware
- ---------------------------------------------------------
- ---------------------------------------------------------
0
5 SOLE VOTING POWER
NUMBER OF
SHARES
BENEFICIALLY
OWNED BY EACH
REPORTING
PERSON WITH
- ---------------------------------------------------------
- ---------------------------------------------------------
0
6 SHARED VOTING POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
7 SOLE DISPOSITIVE 0
POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
8 SHARED DISPOSITIVE 8,207,780
POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
9 AGGREGATE AMOUNT BENEFICIALLY OWNED BY EACH
REPORTING PERSON
8,207,780
- ---------------------------------------------------------
- ---------------------------------------------------------
10 CHECK IF THE AGGREGATE AMOUNT IN ROW (9)
EXCLUDES CERTAIN SHARES (See Instructions)
[ ]
- ---------------------------------------------------------
- ---------------------------------------------------------
11 PERCENT OF CLASS REPRESENTED BY AMOUNT IN ROW (9)
6.31%
- ---------------------------------------------------------
- ---------------------------------------------------------
12 TYPE OF REPORTING PERSON (See Instructions)
HC
- ---------------------------------------------------------
- ---------------------------------------------------------
1 NAMES OF REPORTING PERSONS
I.R.S. IDENTIFICATION NO. OF ABOVE PERSONS
(ENTITIES ONLY):
United States Trust Company, NA
13-3818954
- ---------------------------------------------------------
- ---------------------------------------------------------
2 CHECK THE APPROPRIATE BOX IF A MEMBER OF A GROUP
(See Instructions)
(a) [ ]
(b) [ ]
- ---------------------------------------------------------
- ---------------------------------------------------------
3 SEC USE ONLY
- ---------------------------------------------------------
- ---------------------------------------------------------
4 CITIZENSHIP OR PLACE OF ORGANIZATION
United States
- ---------------------------------------------------------
- ---------------------------------------------------------
0
5 SOLE VOTING POWER
NUMBER OF
SHARES
BENEFICIALLY
OWNED BY EACH
REPORTING
PERSON WITH
- ---------------------------------------------------------
- ---------------------------------------------------------
0
6 SHARED VOTING POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
7 SOLE DISPOSITIVE 0
POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
8 SHARED DISPOSITIVE 8,207,780
POWER
- ---------------------------------------------------------
- ---------------------------------------------------------
9 AGGREGATE AMOUNT BENEFICIALLY OWNED BY EACH
REPORTING PERSON
8,207,780
- ---------------------------------------------------------
- ---------------------------------------------------------
10 CHECK IF THE AGGREGATE AMOUNT IN ROW (9)
EXCLUDES CERTAIN SHARES (See Instructions)
[ ]
- ---------------------------------------------------------
- ---------------------------------------------------------
11 PERCENT OF CLASS REPRESENTED BY AMOUNT IN ROW (9)
6.31%
- ---------------------------------------------------------
- ---------------------------------------------------------
12 TYPE OF REPORTING PERSON (See Instructions)
BK
- ---------------------------------------------------------
Explanatory Note:
This Amendment No. 1 amends the statement on Schedule 13G filed by
United States Trust Company, N.A. with the Securities and Exchange
Commission on February 14, 2007. United States Trust Company, N.A.
merged into Bank of America Corporation on July 1, 2007.
Item 1(a). Name of Issuer:
APPLIED NEUROSOLUTIONS INC.
Item 1(b). Address of Issuer's Principal Executive Offices:
50 LAKEVIEW PKWY, SUITE 111
VERNON HILLS, IL 60661
Item 2(a). Name of Person Filing:
Bank of America Corporation
NB Holdings Corporation
United States Trust Company, N.A.
Item 2(b). Address of Principal Business Office or, if None,
Residence:
Each Reporting Person has its or his principal business
office at 100 North Tryon Street, Floor 25, Bank of America
Corporate Center, Charlotte, NC 28255.
Item 2(c). Citizenship:
Bank of America Corporation Delaware
NB Holdings Corporation Delaware
United States Trust Company, N.A. United States
Item 2(d). Title of Class of Securities:
Common Stock
Item 2(e). CUSIP Number:
03823Y104
Item 3. If This Statement is Filed Pursuant to Rule 13d-1(b), or
13d-2(b) or (c), Check Whether the Person Filing is a:
(a) [ ] Broker or dealer registered under Section 15 of
the Exchange Act.
(b) [ ] Bank as defined in Section 3(a)(6) of the
Exchange Act.
(c) [ ] Insurance company as defined in Section 3(a)(19)
of the Exchange Act.
(d) [ ] Investment company registered under Section 8 of
the Investment Company Act.
(e) [ ] An investment adviser in accordance with
Rule 13d-1(b)(1)(ii)(E).
(f) [ ] An employee benefit plan or endowment fund in
accordance with Rule 13d-1(b)(1)(ii)(F).
(g) [X] A parent holding company or control person in
accordance with Rule 13d-1(b)(1)(ii)(G).
(h) [ ] A savings association as defined in Section 3(b)
of the Federal Deposit Insurance Act.
(i) [ ] A church plan that is excluded from the
definition of an investment company under Section
3(c)(14) of the Investment Company Act.
(j) [ ] Group, in accordance with Rule 13d-1(b)(1)(ii)(J).
If this statement is filed pursuant to Rule 13d-1(c), check
this box. [ ]
Item 4. Ownership:
With respect to the beneficial ownership of the reporting
person, see Items 5 through 11 of the cover pages to this
Schedule 13G, which are incorporated herein by reference.
Item 5. Ownership of Five Percent or Less of a Class:
If this statement is being filed to report the fact that as
of the date hereof the reporting person has ceased to be
the beneficial owner of more than five percent of the class
of securities, check the following [ ].
Item 6. Ownership of More than Five Percent on Behalf of Another
Person:
Not applicable.
Item 7. Identification and Classification of the Subsidiary Which
Acquired the Security Being Reported on by the Parent
Holding Company or Control Person:
With respect to Subsidiary Identification and
Classification, see Items 5 through 11 of the cover pages
to this Schedule 13G, which are incorporated herein by
reference.
Item 8. Identification and Classification of Members of the Group:
Not applicable.
Item 9. Notice of Dissolution of Group:
Not applicable.
Item 10. Certification:
By signing below each of the undersigned certifies that, to
the best of such undersigned's knowledge and belief, the
securities referred to above were acquired and are held in
the ordinary course of business and were not acquired and
are not for the purpose of or with the effect of changing
or influencing the control of the issuer of the securities
and were not acquired and are not held in connection with
or as a participant in any transaction having that purpose
or effect.
SIGNATURE
After reasonable inquiry and to the best of my knowledge and belief,
I certify that the information set forth in this statement is true,
complete and correct.
Dated: February 5, 2008
Bank of America Corporation
NB Holdings Corporation
By: /s/ Lucille E. Reymann
_____________________
Lucille E. Reymann
Senior Vice President
United States Trust Company, N.A.
By: /s/ Michael Murphy
______________________
Michael Murphy
Senior Vice President
Exhibit 99.1
EXHIBIT 99.1 - JOINT FILING AGREEMENT
The undersigned hereby agree that they are filing this
statement jointly pursuant to Rule 13d-1(k)(1). Each of them is
responsible for the timely filing of such Schedule 13G and any
amendments thereto, and for the completeness and accuracy of the
information concerning such person contained therein; but none of
them is responsible for the completeness or accuracy of the
information concerning the other persons making the filing, unless
such person knows or has reason to believe that such information is
inaccurate.
In accordance with Rule 13d-1(k)(1) promulgated under the
Securities and Exchange Act of 1934, as amended, the undersigned
hereby agree to the joint filing with each other on behalf of each
of them of to such a statement on Schedule 13G with respect to the
common stock of beneficially owned by each of them. This Joint
Filing Agreement shall be included as an exhibit to such Schedule
13G.
Dated: February 5, 2008
Bank of America Corporation
NB Holdings Corporation
By: /s/ Lucille E. Reymann
_____________________
Lucille E. Reymann
Senior Vice President
United States Trust Company, N.A.
By: /s/ Michael Murphy
______________________
Michael Murphy
Senior Vice President