FORM 4
Check this box if no longer subject to Section 16. Form 4 or Form 5 obligations may continue. See Instruction 1(b).
UNITED STATES SECURITIES AND EXCHANGE COMMISSION
Washington, D.C. 20549

STATEMENT OF CHANGES IN BENEFICIAL OWNERSHIP OF SECURITIES

Filed pursuant to Section 16(a) of the Securities Exchange Act of 1934 or Section 30(h) of the Investment Company Act of 1940
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(Print or Type Responses)
1. Name and Address of Reporting Person *
BANK OF AMERICA VENTURES
   2. Issuer Name and Ticker or Trading Symbol
ACUSPHERE INC [ACUS] 		5. Relationship of Reporting Person(s) to Issuer
(Check all applicable)
_____ Director __X__ 10% Owner
_____ Officer (give title below) _____ Other (specify below)
(Last)
(First)
(Middle)
, 		 3. Date of Earliest Transaction (Month/Day/Year)
06/10/2004
(Street)

,  		 4. If Amendment, Date Original Filed(Month/Day/Year)
 6. Individual or Joint/Group Filing(Check Applicable Line)
___ Form filed by One Reporting Person
_X_ Form filed by More than One Reporting Person
(City)
(State)
(Zip)
Table I - Non-Derivative Securities Acquired, Disposed of, or Beneficially Owned
1.Title of Security
(Instr. 3)		 2. Transaction Date (Month/Day/Year) 2A. Deemed Execution Date, if any (Month/Day/Year) 3. Transaction Code
(Instr. 8)		 4. Securities Acquired (A) or Disposed of (D)
(Instr. 3, 4 and 5)		 5. Amount of Securities Beneficially Owned Following Reported Transaction(s)
(Instr. 3 and 4)		 6. Ownership Form: Direct (D) or Indirect (I)
(Instr. 4)		 7. Nature of Indirect Beneficial Ownership
(Instr. 4)
Code V Amount (A) or (D) Price
Common Stock               1,314,164 D  
Common Stock               146,016 I See Footnote 1. (1)
Common Stock               452,414 I See Footnote 2. (2)
Common Stock               6,521 I See Footnote 3. (3)

Reminder: Report on a separate line for each class of securities beneficially owned directly or indirectly.
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Table II - Derivative Securities Acquired, Disposed of, or Beneficially Owned
(e.g., puts, calls, warrants, options, convertible securities)
1. Title of Derivative Security
(Instr. 3)		 2. Conversion or Exercise Price of Derivative Security 3. Transaction Date (Month/Day/Year) 3A. Deemed Execution Date, if any (Month/Day/Year) 4. Transaction Code
(Instr. 8)		 5. Number of Derivative Securities Acquired (A) or Disposed of (D)
(Instr. 3, 4, and 5)		 6. Date Exercisable and Expiration Date
(Month/Day/Year)		 7. Title and Amount of Underlying Securities
(Instr. 3 and 4)		 8. Price of Derivative Security
(Instr. 5)		 9. Number of Derivative Securities Beneficially Owned Following Reported Transaction(s)
(Instr. 4)		 10. Ownership Form of Derivative Security: Direct (D) or Indirect (I)
(Instr. 4)		 11. Nature of Indirect Beneficial Ownership
(Instr. 4)
Code V (A) (D) Date Exercisable Expiration Date Title Amount or Number of Shares
Options (Right to Buy) $ 6.14 06/10/2004   A   25,000   07/10/2004(4) 06/10/2014 Common Stock 25,000 $ 6.14 25,000 I See Footnote 5. (5)

Reporting Owners

Reporting Owner Name / Address Relationships
 Director  10% Owner  Officer  Other
BANK OF AMERICA VENTURES

 		     X    
BANK OF AMERICA NA
110 N TYRONE
CHARLOTTE, NC 28255		     X    
NB HOLDINGS CORP
100 NORTH TRYON STREET
CHARLOTTE, NC 28255		     X    
BANK OF AMERICA CORP /DE/
BANK OF AMERICA CORPORATE CENTER
100 N TRYON ST
CHARLOTTE, NC 28255		     X    

Signatures

 By: Kate Mitchell, President and Managing Director, Bank of America Ventures   06/15/2004
**Signature of Reporting Person Date
 By: Charles F. Bowman, Senior Vice President, BANK OF AMERICA, N.A.   06/15/2004
**Signature of Reporting Person Date
 By: Charles F. Bowman, Senior Vice President, NB HOLDINGS CORPORATION   06/15/2004
**Signature of Reporting Person Date
 By: Charles F. Bowman, Senior Vice President, BANK OF AMERICA CORPORATION   06/15/2004
**Signature of Reporting Person Date

Explanation of Responses:

* If the form is filed by more than one reporting person, see Instruction 4(b)(v).
** Intentional misstatements or omissions of facts constitute Federal Criminal Violations. See 18 U.S.C. 1001 and 15 U.S.C. 78ff(a).
(1) Held by BA Venture Partners II, with which the Reporting Persons have an employment releationship. The Reporting Persons disclaim beneficial ownership of such securities.
(2) Held by BancBoston Ventures, Inc. The Reporting Persons disclaim beneficial ownership of such securities.
(3) Held by Bank of America Capital Corporation. The Reporting Persons disclaim beneficial ownership of such securities.
(4) The options vest over four years on a monthly basis beginning on July 10, 2004.
(5) The option was granted to Kate Mitchell, who is President and Managing Director of Bank of America Ventures. Pursuant to the policies of BA Capital Company, L.P and its affiliates, Mr. Sheridan is deemed to hold the reported option for the benefit of BA Capital Company, L.P and must exercise the option solely upon the direction of BA Capital Company, L.P. BA Capital Company, L.P may be deemed the indirect beneficial owner of the option. BA Capital Company, L.P disclaims beneficial ownership of the option except to the extent of its pecuniary interest therein.

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