Ownership Submission

FORM 3

UNITED STATES SECURITIES AND EXCHANGE COMMISSION
Washington, D.C. 20549
INITIAL STATEMENT OF BENEFICIAL OWNERSHIP OF SECURITIES

Filed pursuant to Section 16(a) of the Securities Exchange Act of 1934 or Section 30(h) of the Investment Company Act of 1940

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(Print or Type Responses)

1. Name and Address of Reporting Person ^*

BANK OF AMERICA VENTURES

2. Date of Event Requiring Statement (Month/Day/Year)
10/07/2003

3. Issuer Name and Ticker or Trading Symbol
ACUSPHERE INC [ACUS]

(Last)

(First)

(Middle)

950 TOWER LANE, SUITE 700

4. Relationship of Reporting Person(s) to Issuer
(Check all applicable)

_____ Director	__X__ 10% Owner
_____ Officer (give title below)	_____ Other (specify below)

5. If Amendment, Date Original Filed(Month/Day/Year)

(Street)

FOSTER CITY, CA 94404

6. Individual or Joint/Group Filing(Check Applicable Line)
___ Form filed by One Reporting Person
_X_ Form filed by More than One Reporting Person

(City)

(State)

(Zip)

Table I - Non-Derivative Securities Beneficially Owned

1.Title of Security
(Instr. 4)

2. Amount of Securities Beneficially Owned
(Instr. 4)

3. Ownership Form: Direct (D) or Indirect (I)
(Instr. 5)

4. Nature of Indirect Beneficial Ownership
(Instr. 5)

Reminder: Report on a separate line for each class of securities beneficially owned directly or indirectly.			SEC 1473 (7-02)
	Persons who respond to the collection of information contained in this form are not required to respond unless the form displays a currently valid OMB control number.

Table II - Derivative Securities Beneficially Owned (e.g., puts, calls, warrants, options, convertible securities)

1. Title of Derivative Security (Instr. 4)	2. Date Exercisable and Expiration Date (Month/Day/Year)		3. Title and Amount of Securities Underlying Derivative Security (Instr. 4)		4. Conversion or Exercise Price of Derivative Security	5. Ownership Form of Derivative Security: Direct (D) or Indirect (I) (Instr. 5)	6. Nature of Indirect Beneficial Ownership (Instr. 5)
1. Title of Derivative Security (Instr. 4)	Date Exercisable	Expiration Date	Title	Amount or Number of Shares	4. Conversion or Exercise Price of Derivative Security		6. Nature of Indirect Beneficial Ownership (Instr. 5)
Series C-2 Convertible Preferred Stock	10/07/2003	⁽¹⁾	Common Stock	231,959	$ ⁽³⁾	D
Series D-2 Convertible Preferred Stock	10/07/2003	⁽¹⁾	Common Stock	60,126	$ ⁽⁴⁾	D
Series E-2 Convertible Preferred Stock	10/07/2003	⁽¹⁾	Common Stock	26,794	$ ⁽⁵⁾	D
Series F-2 Convertible Preferred Stock	10/07/2003	⁽¹⁾	Common Stock	1,953	$ ⁽⁶⁾	D
Series I-2 Convertible Preferred Stock	10/07/2003	⁽¹⁾	Common Stock	55,922	$ ⁽⁷⁾	D
Series J-2 Convertible Preferred Stock	10/07/2003	⁽¹⁾	Common Stock	287,235	$ ⁽⁸⁾	D
Series J-3 Convertible Preferred Stock	10/07/2003	⁽¹⁾	Common Stock	325,368	$ ⁽⁹⁾	D
10% Secured Convertible Promissory Note	10/07/2003	06/30/2004⁽²⁾	Series J Convertible Preferred Stock/Common Stock	324,130	$ ⁽¹⁰⁾	D
Warrant to Purchase Series J Convertible Preferred Stock	10/07/2003	04/11/2008	Series J Convertible Preferred Stock/Common Stock	61,811	$ ⁽¹¹⁾	D
Series C-2 Convertible Preferred Stock	10/07/2003	⁽¹⁾	Common Stock	25,773	$ ⁽³⁾	I	See exhibit 99.2 for Explanation of Responses. ⁽¹²⁾
Series D-2 Convertible Preferred Stock	10/07/2003	⁽¹⁾	Common Stock	6,681	$ ⁽⁴⁾	I	See exhibit 99.2 for Explanation of Responses. ⁽¹²⁾
Series E-2 Convertible Preferred Stock	10/07/2003	⁽¹⁾	Common Stock	2,977	$ ⁽⁵⁾	I	See exhibit 99.2 for Explanation of Responses. ⁽¹²⁾
Series F-2 Convertible Preferred Stock	10/07/2003	⁽¹⁾	Common Stock	217	$ ⁽⁶⁾	I	See exhibit 99.2 for Explanation of Responses. ⁽¹²⁾
Series I-2 Convertible Preferred Stock	10/07/2003	⁽¹⁾	Common Stock	6,213	$ ⁽⁷⁾	I	See exhibit 99.2 for Explanation of Responses. ⁽¹²⁾
Series J-2 Convertible Preferred Stock	10/07/2003	⁽¹⁾	Common Stock	31,915	$ ⁽⁸⁾	I	See exhibit 99.2 for Explanation of Responses. ⁽¹²⁾
Series J-3 Convertible Preferred Stock	10/07/2003	⁽¹⁾	Common Stock	36,152	$ ⁽⁹⁾	I	See exhibit 99.2 for Explanation of Responses. ⁽¹²⁾
10% Secured Convertible Promissory Note	10/07/2003	06/30/2004⁽²⁾	Series J Convertible Preferred Stock/Common Stock	36,014	$ ⁽¹⁰⁾	I	See exhibit 99.2 for Explanation of Responses. ⁽¹²⁾
Warrant to Purchase Series J Convertible Preferred Stock	10/07/2003	04/11/2008	Series J Convertible Preferred Stock/Common Stock	6,867	$ ⁽¹¹⁾	I	See exhibit 99.2 for Explanation of Responses. ⁽¹²⁾

Reporting Owners

Reporting Owner Name / Address	Relationships
Reporting Owner Name / Address	Director	10% Owner	Officer	Other
BANK OF AMERICA VENTURES 950 TOWER LANE SUITE 700 FOSTER CITY, CA 94404		X
BANK OF AMERICA CORP /DE/ BANK OF AMERICA CORPORATE CENTER 100 N TRYON ST CHARLOTTE, NC 28255		X
BANK OF AMERICA NA 101 S TRYON ST CHARLOTTE, NC 28255		X

Signatures

Kate Mitchell, President and Managing Director, Bank of America Ventures		10/07/2003
^**Signature of Reporting Person		Date
Pamela Reed, Assistant Vice President, Bank of America Corporation		10/07/2003
^**Signature of Reporting Person		Date
Pamela Reed, Assistant Vice President, Bank of America, N.A.		10/07/2003
^**Signature of Reporting Person		Date
Rachel Cummings, Senior Vice President, NB Holdings Corporation		10/07/2003
^**Signature of Reporting Person		Date

Explanation of Responses:

*	If the form is filed by more than one reporting person, see Instruction 5(b)(v).
**	Intentional misstatements or omissions of facts constitute Federal Criminal Violations. See 18 U.S.C. 1001 and 15 U.S.C. 78ff(a).
(1)	See exhibit 99.2 for Explanation of Responses.
(2)	See exhibit 99.2 for Explanation of Responses.
(3)	See exhibit 99.2 for Explanation of Responses.
(4)	See exhibit 99.2 for Explanation of Responses.
(5)	See exhibit 99.2 for Explanation of Responses.
(6)	See exhibit 99.2 for Explanation of Responses.
(7)	See exhibit 99.2 for Explanation of Responses.
(8)	See exhibit 99.2 for Explanation of Responses.
(9)	See exhibit 99.2 for Explanation of Responses.
(10)	See exhibit 99.2 for Explanation of Responses.
(11)	See exhibit 99.2 for Explanation of Responses.
(12)	See exhibit 99.2 for Explanation of Responses.

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