Ownership Submission

FORM 4

Check this box if no longer subject to Section 16. Form 4 or Form 5 obligations may continue. See Instruction 1(b).

UNITED STATES SECURITIES AND EXCHANGE COMMISSION
Washington, D.C. 20549
STATEMENT OF CHANGES IN BENEFICIAL OWNERSHIP OF SECURITIES

Filed pursuant to Section 16(a) of the Securities Exchange Act of 1934 or Section 30(h) of the Investment Company Act of 1940

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OMB Number:

3235-0287

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(Print or Type Responses)

1. Name and Address of Reporting Person ^*

BANK OF AMERICA VENTURES

2. Issuer Name and Ticker or Trading Symbol
ACUSPHERE INC [ACUS]

5. Relationship of Reporting Person(s) to Issuer
(Check all applicable)

_____ Director	__X__ 10% Owner
_____ Officer (give title below)	_____ Other (specify below)

(Last)

(First)

(Middle)

3. Date of Earliest Transaction (Month/Day/Year)
10/14/2003

(Street)

4. If Amendment, Date Original Filed(Month/Day/Year)

6. Individual or Joint/Group Filing(Check Applicable Line)
___ Form filed by One Reporting Person
_X_ Form filed by More than One Reporting Person

(City)

(State)

(Zip)

Table I - Non-Derivative Securities Acquired, Disposed of, or Beneficially Owned

1.Title of Security (Instr. 3)	2. Transaction Date (Month/Day/Year)	2A. Deemed Execution Date, if any (Month/Day/Year)	3. Transaction Code (Instr. 8)		4. Securities Acquired (A) or Disposed of (D) (Instr. 3, 4 and 5)			5. Amount of Securities Beneficially Owned Following Reported Transaction(s) (Instr. 3 and 4)	6. Ownership Form: Direct (D) or Indirect (I) (Instr. 4)	7. Nature of Indirect Beneficial Ownership (Instr. 4)
1.Title of Security (Instr. 3)	2. Transaction Date (Month/Day/Year)	2A. Deemed Execution Date, if any (Month/Day/Year)	Code	V	Amount	(A) or (D)	Price		6. Ownership Form: Direct (D) or Indirect (I) (Instr. 4)	7. Nature of Indirect Beneficial Ownership (Instr. 4)
Common Stock	10/14/2003		C		1,314,164	A	$ 0	1,314,164	D
Common Stock	10/14/2003		C		146,016	A	$ 0	146,016	I	See Exhibit 99.2 for Explanation of Reponses ⁽¹⁾

Reminder: Report on a separate line for each class of securities beneficially owned directly or indirectly.
	Persons who respond to the collection of information contained in this form are not required to respond unless the form displays a currently valid OMB control number.		SEC 1474 (9-02)

Table II - Derivative Securities Acquired, Disposed of, or Beneficially Owned
(e.g., puts, calls, warrants, options, convertible securities)

1. Title of Derivative Security
(Instr. 3)

2. Conversion or Exercise Price of Derivative Security

3. Transaction Date (Month/Day/Year)

3A. Deemed Execution Date, if any (Month/Day/Year)

4. Transaction Code
(Instr. 8)

5. Number of Derivative Securities Acquired (A) or Disposed of (D)
(Instr. 3, 4, and 5)

6. Date Exercisable and Expiration Date
(Month/Day/Year)

7. Title and Amount of Underlying Securities
(Instr. 3 and 4)

8. Price of Derivative Security
(Instr. 5)

9. Number of Derivative Securities Beneficially Owned Following Reported Transaction(s)
(Instr. 4)

10. Ownership Form of Derivative Security: Direct (D) or Indirect (I)
(Instr. 4)

11. Nature of Indirect Beneficial Ownership
(Instr. 4)

Code

(A)

(D)

Date Exercisable

Expiration Date

Title

Amount or Number of Shares

Series C-2 Convertible Preferred Stock

⁽²⁾

10/14/2003

1,401,869 ⁽²⁾

10/14/2003

⁽¹²⁾

Common Stock

231,959

$ 0 ⁽²⁾

Series D-2 Convertible Preferred Stock

⁽³⁾

10/14/2003

343,385 ⁽³⁾

10/14/2003

⁽¹²⁾

Common Stock

60,126

$ 0 ⁽³⁾

Series E-2 Convertible Preferred Stock

⁽⁴⁾

10/14/2003

151,020 ⁽⁴⁾

10/14/2003

⁽¹²⁾

Common Stock

26,794

$ 0 ⁽⁴⁾

Series F-2 Convertible Preferred Stock

⁽⁵⁾

10/14/2003

10,556 ⁽⁵⁾

10/14/2003

⁽¹²⁾

Common Stock

1,953

$ 0 ⁽⁵⁾

Series I-2 Convertible Preferred Stock

⁽⁶⁾

10/14/2003

110,666 ⁽⁶⁾

10/14/2003

⁽¹²⁾

Common Stock

55,922

$ 0 ⁽⁶⁾

Series J-2 Convertible Preferred Stock

⁽⁷⁾

10/14/2003

1,914,896 ⁽⁷⁾

10/14/2003

⁽¹²⁾

Common Stock

287,235

$ 0 ⁽⁷⁾

Series J-3 Convertible Preferred Stock

⁽⁸⁾

10/14/2003

1,084,559 ⁽⁸⁾

10/14/2003

⁽¹²⁾

Common Stock

325,368

$ 0 ⁽⁸⁾

10% Secured Convertible Promissory Note

⁽⁹⁾

10/14/2003

360,144 ⁽⁹⁾

10/14/2003

⁽¹⁰⁾

Common Stock

324,808

$ 0 ⁽⁹⁾

Warrant to Purchase Series J Convertible Preferred Stock

⁽¹¹⁾

10/14/2003

68,678 ⁽¹¹⁾

10/14/2003

04/11/2008

Common Stock

61,811

$ 0 ⁽¹¹⁾

Series C-2 Convertible Preferred Stock

⁽²⁾

10/14/2003

1,401,869 ⁽²⁾

10/14/2003

⁽¹²⁾

Common Stock

25,773

$ 0 ⁽²⁾

See Exhibit 99.2 for Explanation of Responses. ⁽¹⁾

Series D-2 Convertible Preferred Stock

⁽³⁾

10/14/2003

343,385 ⁽³⁾

10/14/2003

⁽¹²⁾

Common Stock

6,681

$ 0 ⁽³⁾

See Exhibit 99.2 for Explanation of Responses. ⁽¹⁾

Series E-2 Convertible Preferred Stock

⁽⁴⁾

10/14/2003

151,020 ⁽⁴⁾

10/14/2003

⁽¹²⁾

Common Stock

2,977

$ 0 ⁽⁴⁾

See Exhibit 99.2 for Explanation of Responses. ⁽¹⁾

Series F-2 Convertible Preferred Stock

⁽⁵⁾

10/14/2003

10,556 ⁽⁵⁾

10/14/2003

⁽¹²⁾

Common Stock

217

$ 0 ⁽⁵⁾

See Exhibit 99.2 for Explanation of Responses. ⁽¹⁾

Series I-2 Convertible Preferred Stock

⁽⁶⁾

10/14/2003

110,666 ⁽⁶⁾

10/14/2003

⁽¹²⁾

Common Stock

6,213

$ 0 ⁽⁶⁾

See Exhibit 99.2 for Explanation of Responses.

Series J-2 Convertible Preferred Stock

⁽⁷⁾

10/14/2003

1,914,896 ⁽⁷⁾

10/14/2003

⁽¹²⁾

Common Stock

31,915

$ 0 ⁽⁷⁾

See Exhibit 99.2 for Explanation of Responses. ⁽¹⁾

Series J-3 Convertible Preferred Stock

⁽⁸⁾

10/14/2003

1,084,559 ⁽⁸⁾

10/14/2003

⁽¹²⁾

Common Stock

36,152

$ 0 ⁽⁸⁾

See Exhibit 99.2 for Explanation of Responses. ⁽¹⁾

10% Secured Convertible Promissory Note

⁽⁹⁾

10/14/2003

360,144 ⁽⁹⁾

10/14/2003

⁽¹⁰⁾

Common Stock

36,089

$ 0 ⁽¹⁰⁾

See Exhibit 99.2 for Explanation of Responses. ⁽¹⁾

Warrant to Purchase Series J Convertible Preferred Stock

⁽¹¹⁾

10/14/2003

68,678 ⁽¹¹⁾

10/14/2003

04/11/2008

Common Stock

6,867

$ 0 ⁽¹¹⁾

See Exhibit 99.2 for Explanation of Responses. ⁽¹⁾

Reporting Owners

Reporting Owner Name / Address	Relationships
Reporting Owner Name / Address	Director	10% Owner	Officer	Other
BANK OF AMERICA VENTURES		X
BANK OF AMERICA CORP /DE/ BANK OF AMERICA CORPORATE CENTER 100 N TRYON ST CHARLOTTE, NC 28255		X
BANK OF AMERICA NA 100 N TRYON CHARLOTTE, NC 28255		X

Signatures

Bank of America Ventures, by Kate Mitchell, President and Managing Director		10/15/2003
^**Signature of Reporting Person		Date
BANK OF AMERICA CORPORATION, by Pamela Reed, Assistant Vice President		10/15/2003
^**Signature of Reporting Person		Date
BANK OF AMERICA, N.A., by Pamela Reed, Assistant Vice President		10/15/2003
^**Signature of Reporting Person		Date
NB HOLDINGS CORPORATION, by Rachel Cummings, Senior Vice President		10/15/2003
^**Signature of Reporting Person		Date

Explanation of Responses:

*	If the form is filed by more than one reporting person, see Instruction 4(b)(v).
**	Intentional misstatements or omissions of facts constitute Federal Criminal Violations. See 18 U.S.C. 1001 and 15 U.S.C. 78ff(a).
(1)	See Exhibit 99.2 for Explanation of Reponses
(2)	See Exhibit 99.2 for Explanation of Reponses
(3)	See Exhibit 99.2 for Explanation of Reponses
(4)	See Exhibit 99.2 for Explanation of Reponses
(5)	See Exhibit 99.2 for Explanation of Reponses
(6)	See Exhibit 99.2 for Explanation of Reponses
(7)	See Exhibit 99.2 for Explanation of Reponses
(8)	See Exhibit 99.2 for Explanation of Reponses
(9)	See Exhibit 99.2 for Explanation of Reponses
(10)	See Exhibit 99.2 for Explanation of Reponses
(11)	See Exhibit 99.2 for Explanation of Reponses
(12)	See Exhibit 99.2 for Explanation of Reponses

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Potential persons who are to respond to the collection of information contained in this form are not required to respond unless the form displays a currently valid OMB number.